Partial Purification of Phytocystatin from Moringa oleifera lam

Background: Phytocystatins are plants cysteine protease inhibitors (CPIs) that known for their numerous uses in medicine and biotechnology. Methods: Different extraction media which include, Sodium Hydroxide, Hydrochloric acid, Chloride, phosphate buffer distilled Water were used to evaluate flower, leave, root, latex, stem bark of Moringa oleifera inhibitory activity against (Papain enzyme). The extract with higher was concentrated by cold acetone precipitation further subjected partial purification using ammonium sulphate fractionation Hydrophobic chromatography on Phenyl Sepharose column. molecular weight partially purified protein estimated sodium dodecyl (SDS) polyacrylamide gel electrophoresis. Results: extracts from the seeds showed CPI 11.23 units/mg protein. Cold precipitated gave high yield 94.6% 60% inhibition protease. a fifty percent concentration (IC50) 1.22±0.3 mg/ml enzyme 63% inhibition. An 13.5kDa obtained electrophoretic analysis Conclusion: could be good source low Inhibitor Cysteine might useful biotechnology traditional medicinal plants, transgenic crops arrest negative pathogenic over expressions proteases